Article
Rapid determination of rifampin resistance in clinical isolates of Mycobacterium tuberculosis by real-time PCR.
Journal of clinical microbiology - 1 Dec 2005
Kocagoz Tanil, Saribas Zeynep, Alp Alpaslan
Abstract excerpt
Real-time PCR was used to determine rifampin resistance in clinical isolates of Mycobacterium tuberculosis. Ninety-six rifampin-resistant isolates and 23 rifampin-susceptible isolates were included in the study. A 305-bp region covering the 81-bp "rifampin resistance-determining region" of rpoB was amplified. Two hybridization probe pairs that covered the most frequent mutation sites in rpoB, codon regions 526 to...
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