Article
The roles of the essential Asp-48 and highly conserved His-43 elucidated by the pH dependence of the pseudouridine synthase TruB.
Archives of biochemistry and biophysics - 1 Jan 2005
Hamilton Christopher S, Spedaliere Christopher J, Ginter Joy M, Johnston Murray V, Mueller Eugene G
Abstract excerpt
All known pseudouridine synthases have a conserved aspartic acid residue that is essential for catalysis, Asp-48 in Escherichia coli TruB. To probe the role of this residue, inactive D48C TruB was oxidized to generate the sulfinic acid cognate of aspartic acid. The oxidation restored significant but reduced catalytic activity, consistent with the proposed roles of Asp-48 as a nucleophile and general base. The...
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