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Article

Drop-in, drop-out allele-specific PCR: a highly sensitive, single-tube method.

Molecular biotechnology - 1 Nov 2004

Alexander Alice, Subramanian Nivedita, Buxbaum Joel N, Jacobson Daniel R

Abstract excerpt

Allelotyping large numbers of samples by allele-specific polymerase chain reaction (PCR) can be problematic if the DNA samples to be tested are of highly variable concentration. On the one hand, analysis of dilute DNA samples often requires nested PCR to produce a product of sufficient yield to be detectable on ethidium bromide-stained agarose gels. Such two-step assays require additional reagents, are...

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PMID: 15542916
DOI: 10.1385/MB:28:3:171

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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