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Optimizing single-tube nested PCR for enhanced genotyping of single cells and in vitro produced bovine embryos.

Gene - 15 Dec 2024

Macedo Rafael de Arruda Jéssica, da Silva Mendonça Mariana, Maravilha Braga Paulo Vitor, Dos Santos Nascimento Leticia, Burla Dias Angelo José, Lopes Rios Álvaro Fabrício, Magnelli Mangiavacchi Paula

Abstract excerpt

Single-tube nested PCR (STnPCR) is a technique that improves nested PCR, reducing potential contamination and false-positive results, enhancing the amplification sensitivity. Despite being commonly used for the detection of microorganisms, STnPCR can be a valuable tool for bovine genotyping, encompassing essential targets as ROSA26 and TSPY, pivotal in the fields of animal reproduction, genetic improvement, and...

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Identifiers

PMID: 39127412
DOI: 10.1016/j.gene.2024.148838

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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