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Article

Directed termination PCR: a one-step approach to mutation detection.

Nucleic acids research - 15 Mar 1998

Chen J, Hebert P D

Abstract excerpt

We describe a novel PCR-based method that allows the generation of nested termination fragments by integrating both selective DNA amplification and directed chain termination into a single PCR reaction. These termination fragments can be examined for sequence variation in either denaturing or non-denaturing polyacrylamide gels. This method provides a one-step and highly effective approach for the detection of...

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Share to DNA, Mitochondrial
Share to DNA Mutational Analysis
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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Share to Point Mutation

Share to Polymerase Chain Reaction

Share to Polymorphism, Single-Stranded Conformational

Share to Sequence Deletion

Source

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Identifiers

PMID: 9490805
DOI: 10.1093/nar/26.6.1546
PMCID: PMC147424

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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