Identification of the structural and functional domains of the large serine recombinase TnpX from Clostridium perfringens.

doi:10.1074/jbc.M409702200

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Article

Identification of the structural and functional domains of the large serine recombinase TnpX from Clostridium perfringens.

The Journal of biological chemistry - 28 Jan 2005

Lucet Isabelle S, Tynan Fleur E, Adams Vicki, Rossjohn Jamie, Lyras Dena, Rood Julian I

Abstract excerpt

Members of the large serine resolvase family of site-specific recombinases are responsible for the movement of several mobile genetic elements; however, little is known regarding the structure or function of these proteins. TnpX is a serine recombinase that is responsible for the movement of the chloramphenicol resistance elements of the Tn4451/3 family. We have shown that TnpX binds differentially to its...

Topics

Bacterial Proteins
Blotting, Southern
Blotting, Western
Catalytic Domain
Chymotrypsin
Circular Dichroism
Cloning, Molecular
Clostridium perfringens
DNA
DNA Nucleotidyltransferases

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Dimerization

Electrophoresis, Polyacrylamide Gel

Mutation

Protein Binding

Protein Structure, Tertiary

Recombinases

Time Factors

Source

Open in PubMed

Identifiers

PMID: 15542858
DOI: 10.1074/jbc.M409702200

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