Article
Two distinct regions of the large serine recombinase TnpX are required for DNA binding and biological function.
Molecular microbiology - 1 May 2006
Adams Vicki, Lucet Isabelle S, Tynan Fleur E, Chiarezza Martina, Howarth Pauline M, Kim Jonathan, Rossjohn Jamie, Lyras Dena, Rood Julian I
Abstract excerpt
The large serine recombinase, TnpX, from the Clostridium perfringens integrative mobilizable element Tn4451, consists of three domains and has two known DNA binding regions. In this study random and site-directed mutagenesis was used to identify other regions of TnpX that were required for biological activity. Genetic and biochemical analysis of these mutants led to the identification of important TnpX residues...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.