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Article

Gene cloning and biochemical characterizations of thermostable ribonuclease HIII from Bacillus stearothermophilus.

Bioscience, biotechnology, and biochemistry - 1 Oct 2004

Chon Hyongi, Nakano Rikita, Ohtani Naoto, Haruki Mitsuru, Takano Kazufumi, Morikawa Masaaki, Kanaya Shigenori

Abstract excerpt

The gene encoding RNase HIII from the thermophilic bacterium Bacillus stearothermophilus was cloned and overexpressed in Escherichia coli, and the recombinant protein (Bst-RNase HIII) was purified and biochemically characterized. Bst-RNase HIII is a monomeric protein with 310 amino acid residues, and shows an amino acid sequence identity of 47.1% with B. subtilis RNase HIII (Bsu-RNase HIII). The enzymatic...

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PMID: 15502360
DOI: 10.1271/bbb.68.2138

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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