Article
Destabilization of psychrotrophic RNase HI in a localized fashion as revealed by mutational and X-ray crystallographic analyses.
The FEBS journal - 1 Jan 2009
Rohman Muhammad S, Tadokoro Takashi, Angkawidjaja Clement, Abe Yumi, Matsumura Hiroyoshi, Koga Yuichi, Takano Kazufumi, Kanaya Shigenori
Abstract excerpt
The Arg97 --> Gly and Asp136 --> His mutations stabilized So-RNase HI from the psychrotrophic bacterium Shewanella oneidensis MR-1 by 5.4 and 9.7 degrees C, respectively, in T(m), and 3.5 and 6.1 kJ x mol(-1), respectively, in DeltaG(H2O). These mutations also stabilized the So-RNase HI derivative (4x-RNase HI) with quadruple thermostabilizing mutations in an additive manner. As a result, the resultant sextuple...
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