Article
Selective suppression of the catalytic activity of cDNA-expressed cytochrome P4502B1 toward polycyclic hydrocarbons in the microsomal membrane: modification of this effect by specific amino acid substitutions.
Biochemistry - 17 Mar 1992
Christou M, Mitchell M J, Aoyama T, Gelboin H V, Gonzalez F J, Jefcoate C R
Abstract excerpt
Human hepatoma HEPG2 cells were infected with recombinant vaccinia virus vectors containing cDNAs encoding both known and variant rat cytochromes P450 (CYP). CYP2B1 and CYP2B2 cytochromes were equally well expressed (110-140 pmol/mg of microsomal protein) and catalyzed metabolism of 7,12-dimethyl...
Topics
- 9,10-Dimethyl-1,2-benzanthracene
- Amino Acids
- Benzo(a)pyrene
- Catalysis
- Cytochrome P-450 Enzyme Inhibitors
- Cytochrome P-450 Enzyme System
- DNA
- Genes, Viral
- Humans
- Intracellular Membranes
- Microsomes, Liver
- Mutation
- Polycyclic Compounds