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Generating conditional gene knockouts in Plasmodium – a toolkit to produce stable DiCre recombinase-expressing parasite lines using CRISPR/Cas9

14 Jun 2017

Abstract excerpt

Successful establishment of CRISPR/Cas9 genome editing technology in Plasmodium spp. has provided a powerful tool to transform Plasmodium falciparum into a genetically more tractable organism. Conditional gene regulation approaches are required to study the function of gene products critical for growth and erythrocyte invasion of blood stage parasites. Here we employ CRISPR/Cas9 to facilitate use of the...

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CRISPR and Genetic Engineering
Malaria Research and Control
Mosquito-borne diseases and control

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 28634346
DOI: 10.1038/s41598-017-03984-3

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