Article
Detection of point mutation in bla T genes of Enterobacteriaceae by biotinylated oligonucleotide probes using microwell hybridization and enzymofluorometric method.
Molecular and cellular probes - 1 Feb 1992
Tham T N, Guesdon J L
Abstract excerpt
Point mutation in the nucleotide sequence of the structural genes for the TEM-type penicillinases can broaden their substrate spectrum towards all beta-lactams except cephamicins and imipenem. We describe here hybridization techniques for the detection of point mutations by non-radioactive oligon...
Topics
- Adsorption
- Base Sequence
- DNA, Bacterial
- Enterobacteriaceae
- Fluorometry
- Genes, Bacterial
- Molecular Sequence Data
- Mutation
- Nucleic Acid Hybridization
- Oligonucleotide Probes