Article
Flow cytometric analysis of the in situ accessibility of Escherichia coli 16S rRNA for fluorescently labeled oligonucleotide probes.
Applied and environmental microbiology - 1 Dec 1998
Fuchs B M, Wallner G, Beisker W, Schwippl I, Ludwig W, Amann R
Abstract excerpt
In situ identification of whole fixed bacterial cells by hybridization with fluorescently labeled, rRNA-targeted oligonucleotide probes is often limited by low signal intensities. In addition to an impermeability of the cell periphery and a low cellular rRNA content, the three-dimensional structu...
Topics
- Base Sequence
- Conserved Sequence
- DNA, Ribosomal
- Escherichia coli
- Evolution, Molecular
- Flow Cytometry
- Fluorescent Dyes
- Genetic Variation
- Models, Molecular
- Nucleic Acid Conformation
- Nucleic Acid Hybridization
- Oligonucleotide Probes
- Operon
- RNA, Bacterial
- RNA, Ribosomal, 16S