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Article

Flow cytometric analysis of the in situ accessibility of Escherichia coli 16S rRNA for fluorescently labeled oligonucleotide probes.

Applied and environmental microbiology - 1 Dec 1998

Fuchs B M, Wallner G, Beisker W, Schwippl I, Ludwig W, Amann R

Abstract excerpt

In situ identification of whole fixed bacterial cells by hybridization with fluorescently labeled, rRNA-targeted oligonucleotide probes is often limited by low signal intensities. In addition to an impermeability of the cell periphery and a low cellular rRNA content, the three-dimensional structure of the ribosome may hinder the access of oligonucleotides to their target sites. Until now, a systematic study on...

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Identifiers

PMID: 9835591
DOI: 10.1128/AEM.64.12.4973-4982.1998
PMCID: PMC90951

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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