Article
Detection of mutations in Mycobacterium tuberculosis genome determining resistance to fluoroquinolones by hybridization on biological microchips.
Bulletin of experimental biology and medicine - 1 Jan 2008
Antonova O V, Gryadunov D A, Lapa S A, Kuz'min A V, Larionova E E, Smirnova T G, Nosova E Y, Skotnikova O I, Chernousova L N, Moroz A M, Zasedatelev A S, Mikhailovich V M
Abstract excerpt
We developed a method of identification of Mycobacterium tuberculosis with simultaneous evaluation of the sensitivity to fluoroquinolones on a biological microchip array. The method of multiplex two-staged PCR followed by hybridization of a biochip makes it possible to detect 8 mutant variants of gyrA gene occurring in fluoroquinolone-resistant strains (approximately 85% all resistant forms) within 1 day. Using...
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