Article
Protein turnover, growth and proliferation in CHO cells. Variation within and between mutant classes for salvage pathway enzymes.
The Biochemical journal - 15 Feb 1992
Gunn J M, Brancheau M R
Abstract excerpt
We have examined the clonal variation in rates of amino acid transport, protein synthesis, protein degradation, growth and proliferation for CHO cells with mutations in the purine and pyrimidine salvage pathways. First we compared three clonal cell lines, each with a different mutation, with the heterozygous parental line AT3-2. Overall, the correlation between rates of protein turnover, growth and proliferation...
Topics
- Adenine Phosphoribosyltransferase
- Animals
- CHO Cells
- Cell Division
- Clone Cells
- Cricetinae
- Culture Media
- Dexamethasone
- Genetic Variation
- Insulin
- Kinetics
- Leucine