Article
Isolation and characterization of Chinese hamster ovary cell mutants defective in glucose transport.
Biochimica et biophysica acta - 9 Mar 1990
Hasegawa K, Anraku Y, Kasahara M, Akamatsu Y, Nishijima M
Abstract excerpt
Cultured Chinese hamster ovary (CHO) cells possess an insulin-sensitive facilitated diffusion system for glucose transport. Mutant clones of CHO cells defective in glucose transport were obtained by repeating the selection procedure, which involved mutagenesis with ethyl methanesulfonate, radiati...
Topics
- Animals
- Autoradiography
- Biological Transport
- Cell Division
- Cell Line
- Cell Separation
- Cricetinae
- Cricetulus
- Deoxyglucose
- Ethyl Methanesulfonate
- Female
- Gene Expression
- Glucose
- Immunoblotting
- Insulin
- Monosaccharide Transport Proteins
- Mutation
- Ovary