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Article

Phenotypic selection and characterization of randomly produced non-haemolytic mutants of the toxic sea anemone protein sticholysin II.

FEBS letters - 24 Sept 2004

Alegre-Cebollada Jorge, Lacadena Valle, Oñaderra Mercedes, Mancheño José M, Gavilanes José G, del Pozo Alvaro Martínez

Abstract excerpt

A rapid screening method for haemolytic activity, using blood agar plates, has been developed to analyze randomly produced mutant variants of the pore-forming protein sticholysin II (Stn II). Those exhibiting a reduced activity were selected and the DNA corresponding to each Stn II variant sequenced. Once the mutation produced was determined, protein variants were isolated and characterized in terms of structure...

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Identifiers

PMID: 15388326
DOI: 10.1016/j.febslet.2004.08.031

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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