Article
Mutations affecting pore formation by haemolysin from Escherichia coli.
Molecular & general genetics : MGG - 1 Apr 1991
Ludwig A, Schmid A, Benz R, Goebel W
Abstract excerpt
By introduction of site-specific deletions, three regions in HlyA were identified, which appear to be involved in pore formation by Escherichia coli haemolysin. Deletion of amino acids 9-37 at the N-terminus led to a haemolysin which had an almost threefold higher specific activity than wild-type...
Topics
- Amino Acid Sequence
- Animals
- Bacterial Proteins
- Base Sequence
- Cattle
- Chromosome Deletion
- DNA, Bacterial
- Electric Conductivity
- Electrophoresis, Polyacrylamide Gel
- Erythrocytes
- Escherichia coli
- Escherichia coli Proteins
- Hemolysin Proteins
- Lipid Bilayers
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Protein Conformation