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Article

Silent mutations at the 5'-end of the cDNA of actinoporins from the sea anemone Stichodactyla helianthus allow their heterologous overproduction in Escherichia coli.

Journal of biotechnology - 1 Jan 2007

Alegre-Cebollada Jorge, Clementi Giorgia, Cunietti Michela, Porres Christian, Oñaderra Mercedes, Gavilanes José G, Pozo Alvaro Martínez Del

Abstract excerpt

Wild-type actinoporins StnI and StnII from the sea anemone Stichodactyla helianthus, as well as their NH(2)-terminal six-His tagged versions, have been overproduced in Escherichia coli. Overproduction of both wild-type proteins was only possible after introducing silent mutations within the 5'-end of their original cDNA sequences. These mutations would prevent the formation of RNA secondary structures blocking...

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PMID: 16930756
DOI: 10.1016/j.jbiotec.2006.07.006

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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