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Article

Depth of proteome issues: a yeast isotope-coded affinity tag reagent study.

Molecular & cellular proteomics : MCP - 1 Jul 2004

Parker Kenneth C, Patterson Dale, Williamson Brian, Marchese Jason, Graber Armin, He Feng, Jacobson Allan, Juhasz Peter, Martin Stephen

Abstract excerpt

As a test case for optimizing how to perform proteomics experiments, we chose a yeast model system in which the UPF1 gene, a protein involved in nonsense-mediated mRNA decay, was knocked out by homologous recombination. The results from five complete isotope-coded affinity tag (ICAT) experiments were combined, two using matrix-assisted laser desorption/ionization (MALDI) tandem mass spectrometry (MS/MS) and three...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Share to Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Source

Identifiers

PMID: 15047794
DOI: 10.1074/mcp.M300110-MCP200

Genes

UPF1

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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