Article
Loss of one asparagine-linked oligosaccharide from human transferrin receptors results in specific cleavage and association with the endoplasmic reticulum.
The Journal of biological chemistry - 5 Mar 1992
Hoe M H, Hunt R C
Abstract excerpt
The coding sequence for the human transferrin receptor gene has been mutated in order to abolish the attachment of the oligosaccharide closest to the transmembrane sequence. Expression of the mutant receptor in Chinese hamster ovary cells resulted, after analysis under nonreducing conditions, in...
Topics
- Animals
- Asparagine
- Base Sequence
- CHO Cells
- Cell Line
- Cricetinae
- Electrophoresis, Polyacrylamide Gel
- Fluorescent Antibody Technique
- Gene Expression
- Hexosaminidases
- Humans
- Molecular Sequence Data
- Mutation
- Oligosaccharides
- Precipitin Tests
- Protein Biosynthesis
- RNA, Messenger
- Receptors, Transferrin