Article
Evaluation of the role of N-linked oligosaccharides in rat placental lactogen action by site-directed mutagenesis.
Endocrine journal - 1 Oct 1998
Hattori N, Nukada T, Oda M, Tanaka S, Ogawa T, Shiota K
Abstract excerpt
To evaluate the role of N-linked oligosaccharides in the molecular action of rat placental lactogen (PL), recombinant PL-Im (recPL-Im) and three recPL-Im mutants were produced in COS-7 cells. The mutants, carrying Gln substitutions of Asn at putative N-glycosylation sites, were generated via site-directed mutagenesis, i.e. two single mutants (N79Q, N128Q) and one double mutant (N79Q/N128Q). Western blot analysis...
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