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Article

4-cyanopyridine, a versatile spectroscopic probe for cytochrome P450 BM3.

The Journal of biological chemistry - 19 Nov 2004

Ost Tobias W B, Clark Jonathan P, Anderson J L Ross, Yellowlees Lesley J, Daff Simon, Chapman Stephen K

Abstract excerpt

The nitrogenous pi -acceptor ligand 4-cyanopyridine (4CNPy) exhibits reversible ligation to ferrous heme in the flavocytochrome P450 BM3 (Kd=1.8 microm for wild type P450 BM3) via its pyridine ring nitrogen. The reduced P450-4CNPy adduct displays unusual spectral properties that provide a useful spectroscopic handle to probe particular aspects of this P450. 4CNPy is competitively displaced upon substrate binding,...

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Identifiers

PMID: 15364917
DOI: 10.1074/jbc.M408601200

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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