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Article

Key mutations alter the cytochrome P450 BM3 conformational landscape and remove inherent substrate bias.

The Journal of biological chemistry - 30 Aug 2013

Butler Christopher F, Peet Caroline, Mason Amy E, Voice Michael W, Leys David, Munro Andrew W

Abstract excerpt

Cytochrome P450 monooxygenases (P450s) have enormous potential in the production of oxychemicals, due to their unparalleled regio- and stereoselectivity. The Bacillus megaterium P450 BM3 enzyme is a key model system, with several mutants (many distant from the active site) reported to alter substrate selectivity. It has the highest reported monooxygenase activity of the P450 enzymes, and this catalytic efficiency...

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Identifiers

PMID: 23828198
DOI: 10.1074/jbc.M113.479717
PMCID: PMC3757202

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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