Article
Alanine-scanning mutagenesis of the beta-sheet region of phage T4 lysozyme suggests that tertiary context has a dominant effect on beta-sheet formation.
Protein science : a publication of the Protein Society - 1 Oct 2004
He Molly M, Wood Zachary A, Baase Walter A, Xiao Hong, Matthews Brian W
Abstract excerpt
In general, alpha-helical conformations in proteins depend in large part on the amino acid residues within the helix and their proximal interactions. For example, an alanine residue has a high propensity to adopt an alpha-helical conformation, whereas that of a glycine residue is low. The sequence preferences for beta-sheet formation are less obvious. To identify the factors that influence beta-sheet...
Topics
- Alanine
- Amino Acid Sequence
- Bacteriophage T4
- Crystallography, X-Ray
- Models, Molecular
- Molecular Sequence Data
- Muramidase
- Mutagenesis
- Mutation
- Protein Folding
- Protein Structure, Secondary
- Protein Structure, Tertiary
- Thermodynamics