An efficient phage plaque screen for the random mutational analysis of the interaction of HIV-1 gp120 with human CD4.

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Article

An efficient phage plaque screen for the random mutational analysis of the interaction of HIV-1 gp120 with human CD4.

The Journal of biological chemistry - 5 May 1992

Tsui P, Sweet R W, Sathe G, Rosenberg M

Abstract excerpt

A lambda phage expression methodology was adapted to dissect protein/ligand interactions efficiently through the creation and rapid screening of large numbers of mutants. Here we describe the method and its specific application to the interaction between the external envelope glycoprotein of the...

Topics

Amino Acid Sequence
Bacteriophage lambda
Binding, Competitive
CD4 Antigens
Genes, Viral
Genetic Vectors
HIV Envelope Protein gp120
HIV-1
Molecular Sequence Data
Mutation
Protein Conformation
Radioimmunoassay
Viral Plaque Assay

Source

Open in PubMed

Identifiers

PMID: 1533631

Genes

CDR2

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-1533631-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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