Article
The genetic analysis of the HIV envelope binding domain on CD4.
Annals of the New York Academy of Sciences - 1 Jan 1990
Arthos J, Deen K C, Shatzman A, Truneh A, Rosenberg M, Sweet R W
Abstract excerpt
Through mutagenesis, we identified a single high-affinity binding site for gp120 on the human CD4 protein. This site is localized in the V1 domain within residues 41 to 55. The collection of mutants was also used to define the epitopes for 55 anti-CD4 monoclonal antibodies. The locations of these epitopes are consistent with a V kappa-like structure for the V1 domain. In the context of this structure, the gp120...
Topics
- Antibodies, Monoclonal
- Binding Sites
- CD4 Antigens
- HIV
- HIV Envelope Protein gp120
- Humans
- Immunoglobulin Variable Region
- Mutation
- Protein Conformation
- Solubility