Article
Structural and kinetic studies on ligand binding in wild-type and active-site mutants of penicillin acylase.
Protein engineering, design & selection : PEDS - 1 May 2004
Alkema Wynand B L, Hensgens Charles M H, Snijder Harm J, Keizer Evelien, Dijkstra Bauke W, Janssen Dick B
Abstract excerpt
Penicillin acylase catalyses the condensation of Calpha-substituted phenylacetic acids with beta-lactam nucleophiles, producing semi-synthetic beta-lactam antibiotics. For efficient synthesis a low affinity for phenylacetic acid and a high affinity for Calpha-substituted phenylacetic acid derivatives is desirable. We made three active site mutants, alphaF146Y, betaF24A and alphaF146Y/betaF24A, which all had a 2-...
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