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Article

A highly active adipyl-cephalosporin acylase obtained via rational randomization.

The FEBS journal - 1 Nov 2007

Otten Linda G, Sio Charles F, Reis Carlos R, Koch Gudrun, Cool Robbert H, Quax Wim J

Abstract excerpt

There is strong interest in creating an enzyme that can deacylate natural cephalosporins such as cephalosporin C in order to efficiently acquire the starting compound for the industrial production of semisynthetic cephalosporin antibiotics. In this study, the active site of the glutaryl acylase from Pseudomonas SY-77 was randomized rationally. Several mutations that were found in previous studies to enhance the...

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PMID: 17922842
DOI: 10.1111/j.1742-4658.2007.06081.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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