Article
Characterization of the beta-lactam binding site of penicillin acylase of Escherichia coli by structural and site-directed mutagenesis studies.
Protein engineering - 1 Dec 2000
Alkema W B, Hensgens C M, Kroezinga E H, de Vries E, Floris R, van der Laan J M, Dijkstra B W, Janssen D B
Abstract excerpt
The binding of penicillin to penicillin acylase was studied by X-ray crystallography. The structure of the enzyme-substrate complex was determined after soaking crystals of an inactive betaN241A penicillin acylase mutant with penicillin G. Binding of the substrate induces a conformational change, in which the side chains of alphaF146 and alphaR145 move away from the active site, which allows the enzyme to...
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