Deuterium exchange of operator 8CH groups as a Raman probe of repressor recognition: interactions of wild-type and mutant lambda repressors with operator OL1.

doi:10.1021/bi00127a012

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Article

Deuterium exchange of operator 8CH groups as a Raman probe of repressor recognition: interactions of wild-type and mutant lambda repressors with operator OL1.

Biochemistry - 31 Mar 1992

Reilly K E, Becka R, Thomas G J

Abstract excerpt

The rate of deuterium exchange of a purine 8CH group in DNA is highly sensitive to both macromolecular secondary structure and intermolecular interactions which restrict solvent access to the major groove [Lamba, O.P., Becka, R., & Thomas, G.J., Jr. (1990) Biopolymers 29, 1465-1477]. We have exploited the sensitivity of the 8CH----8CD reaction to probe DNA recognition by the helix-turn-helix (HTH) motif of phage...

Topics

Adenine
Adenine Nucleotides
Amino Acid Sequence
Bacteriophage lambda
Base Sequence
DNA Probes
DNA-Binding Proteins
Deuterium
Guanine
Guanine Nucleotides
Kinetics

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Molecular Sequence Data

Mutation

Nucleic Acid Conformation

Operator Regions, Genetic

Purines

Repressor Proteins

Spectrum Analysis, Raman

Source

Open in PubMed

Identifiers

PMID: 1532510
DOI: 10.1021/bi00127a012

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1532510-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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