Article
31P nuclear magnetic resonance spectra and dissociation constants of lac repressor headpiece.duplex operator complexes: the importance of phosphate backbone flexibility in protein.DNA recognition.
Biochemistry - 13 Jul 1993
Botuyan M V, Keire D A, Kroen C, Gorenstein D G
Abstract excerpt
An alkaline phosphatase assay was used to determine the dissociation constants (KD) of the lac repressor N-terminal 56 amino acid fragment of the wild type and of a Y7I mutant complexed to 22 base pair (bp) wild-type and mutant symmetrical operator sequences. KD's in 0.35 M monovalent salt ranged...
Topics
- Alkaline Phosphatase
- Base Sequence
- DNA
- DNA-Binding Proteins
- Esters
- Magnetic Resonance Spectroscopy
- Molecular Sequence Data
- Mutation
- Operator Regions, Genetic
- Phosphorus Isotopes
- Repressor Proteins
- Sodium Chloride
- Temperature