Design of the helix-turn-helix motif: nonlocal effects of quaternary structure in DNA recognition investigated by laser Raman spectroscopy.

doi:10.1021/bi00232a003

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Back to search

Article

Design of the helix-turn-helix motif: nonlocal effects of quaternary structure in DNA recognition investigated by laser Raman spectroscopy.

Biochemistry - 7 May 1991

Benevides J M, Weiss M A, Thomas G J

Abstract excerpt

The operator-binding domain of phage lambda repressor provides a model for DNA recognition by the helix-turn-helix (HTH) motif. In the wild-type protein, dimerization is mediated by hydrophobic packing (of the dyad-related helix 5), which serves as an indirect determinant of operator affinity. The mutant repressor, Tyr88----Cys, forms an intersubunit disulfide linkage and exhibits enhancement of both structural...

Topics

DNA
DNA-Binding Proteins
Disulfides
Hydrogen Bonding
Lasers
Magnetic Resonance Spectroscopy
Mutation
Operator Regions, Genetic
Protein Conformation
Repressor Proteins
Solubility

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Spectrum Analysis, Raman

Tyrosine

Vibration

Viral Proteins

Viral Regulatory and Accessory Proteins

X-Ray Diffraction

Source

Open in PubMed

Identifiers

PMID: 2021630
DOI: 10.1021/bi00232a003

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2021630-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Design of the helix-turn-helix motif: nonlocal effects of quaternary structure in DNA recognition investigated by laser Raman spectroscopy.PMID 2021630Read ↗

An altered specificity mutation in the lambda repressor induces global reorganization of the protein-DNA interface.Semantic proximity Read ↗

Deuterium exchange of operator 8CH groups as a Raman probe of repressor recognition: interactions of wild-type and mutant lambda repressors with operator OL1.Semantic proximity Read ↗

Nonresonance Raman difference spectroscopy: a general probe of protein structure, ligand binding, enzymatic catalysis, and the structures of other biomacromolecules.Semantic proximity Read ↗

The structural basis for enhanced stability and reduced DNA binding seen in engineered second-generation Cro monomers and dimers.Semantic proximity Read ↗

AV77 hinge mutation stabilizes the helix-turn-helix domain of trp repressor.Semantic proximity Read ↗

Probing the physical basis for trp repressor-operator recognition.Semantic proximity Read ↗

The DNA binding arm of lambda repressor: critical contacts from a flexible region.Semantic proximity Read ↗

Surface plasmon resonance studies of wild-type and AV77 tryptophan repressor resolve ambiguities in super-repressor activity.Semantic proximity Read ↗

Isolation of lambda repressor mutants with defects in cooperative operator binding.Semantic proximity Read ↗

A study of energetics of cooperative interaction using a mutant lambda-repressor.Semantic proximity Read ↗

Anatomy of specific interactions between lambda repressor and operator DNA.Semantic proximity Read ↗

The solution structure and dynamics of an Arc repressor mutant reveal premelting conformational changes related to DNA binding.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.