Overexpression of the E1B 55-kilodalton (482R) protein of human adenovirus type 12 appears to permit efficient transformation of primary baby rat kidney cells in the absence of the E1B 19-kilodalton protein.

Abstract excerpt

To analyze the structure and function of the E1B 19,000-molecular-weight protein (19K protein) (163R) of human adenovirus type 12, mutants were produced at various positions across the 163R-coding sequence. Viruses bearing mutations within the first 100 or so amino acids yielded unstable 163R-related products, induced DNA degradation and enhanced cytopathic effect (cyt/deg phenotype) in KB cells, and transformed...

Topics

• Adenovirus Early Proteins
• Adenoviruses, Human
• Animals
• Blotting, Northern
• Cell Line
• Cell Transformation, Viral
• DNA
• Electrophoresis, Polyacrylamide Gel
• Gene Expression Regulation, Viral
• Genetic Complementation Test