Article
An yeast nuclear mutation conferring temperature-sensitivity to the mitochondrial tryptophanyl-tRNA synthetase.
Current genetics - 1 Apr 1992
Entrup R, Langgut W, Lisowsky T, Schweizer E
Abstract excerpt
The conditional respiratory-deficient Saccharomyces cerevisiae mutant pet-ts2281 was complemented by an yeast genomic DNA library. The gene thus isolated was sequenced and proved to be identical to the known MSW1 sequence encoding mitochondrial tryptophanyl-tRNA synthetase (Myers and Tzagoloff 1985). Compared to the wild-type, the ts2281 mutant allele of MSW1 contained a single T----C transition leading to a...
Topics
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- Mitochondria
- Molecular Sequence Data
- Mutation
- Saccharomyces cerevisiae
- Temperature
- Tryptophan-tRNA Ligase