Article
Cytoplasmic tyrosyl-tRNA synthetase rescues the defect in mitochondrial genome maintenance caused by the nuclear mutation mgm104-1 in the yeast Saccharomyces cerevisiae.
Molecular & general genetics : MGG - 1 Aug 1997
Guan M X
Abstract excerpt
The yeast nuclear mutation mgm104-1, which leads to slow growth on glucose medium and temperature-sensitive (ts) loss of mitochondrial DNA (mtDNA), has been identified by screening a collection of temperature-sensitive mutants on glycerol medium. A nuclear gene was isolated from a genomic DNA lib...
Topics
- Base Sequence
- Cell Nucleus
- Cloning, Molecular
- Cytoplasm
- DNA Primers
- DNA, Fungal
- DNA, Mitochondrial
- Genes, Fungal
- Genetic Complementation Test
- Genome, Fungal
- Molecular Sequence Data
- Mutation
- Phenotype
- Polymerase Chain Reaction
- Saccharomyces cerevisiae
- Temperature
- Tyrosine-tRNA Ligase