Exploring the Mechanism of β-Lactam Ring Protonation in the Class A β-lactamase Acylation Mechanism Using Neutron and X-ray Crystallography.

Abstract excerpt

The catalytic mechanism of class A β-lactamases is often debated due in part to the large number of amino acids that interact with bound β-lactam substrates. The role and function of the conserved residue Lys 73 in the catalytic mechanism of class A type β-lactamase enzymes is still not well understood after decades of scientific research. To better elucidate the functions of this vital residue, we used both...

Topics

• Acylation
• Anti-Bacterial Agents
• Catalysis
• Catalytic Domain
• Cefotaxime
• Crystallography, X-Ray
• Drug Design
• Enzyme Inhibitors
• Models, Molecular
• Mutation