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Article

Cysteine-scanning mutagenesis of the fifth external loop of serotonin transporter.

Biochemistry - 6 Jul 2004

Keller Preston C, Stephan Megan, Glomska Hanna, Rudnick Gary

Abstract excerpt

External loop 5 (EL5) of serotonin transporter was analyzed by mutating each of the residues from Thr-480 to Ala-511, one at a time, with cysteine. Cysteine was well-tolerated at most positions, although G485C, Y495C, and E508C had low transport activities. Replacement with cysteine rendered mutants G484C-P499C sensitive to partial or complete inactivation by [2-(trimethylammonium)ethyl] methanethiosulfonate and...

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PMID: 15222762
DOI: 10.1021/bi035971g

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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