Article
Transforming activity and the level of Tax protein: effect of one point mutation in HTLV-I tax gene.
International journal of cancer - 9 Sept 1992
Sakurai M, Yamaoka S, Nosaka T, Akayama M, Tanaka A, Maki M, Hatanaka M
Abstract excerpt
The transcriptional trans-activator molecule of HTLV-I, Tax, is known to transform rodent fibroblasts. A revertant clone expressing Tax was obtained by treating transformed Rat-I cells harboring a single copy of the tax gene with a mutagen. Sequence analysis of the tax gene of the revertant clone revealed that it had one point mutation at codon 12(CTT----TTT), resulting in a change from Leu to Phe. The...
Topics
- Amino Acid Sequence
- Animals
- Cell Transformation, Viral
- Colony-Forming Units Assay
- Gene Products, tax
- Genes, pX
- Human T-lymphotropic virus 1
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction