Article
[Rapid detection of rpoB mutations in Mycobacterium tuberculosis by gene array].
Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases - 1 May 2004
Cao Li-xue, Wu Xue-qiong, Liang Jian-qin, Li Hong-min, Zhang Jun-xian
Abstract excerpt
OBJECTIVE: To develop a new method, gene array, which can be used for rapid detection of rpoB mutations in Mycobacterium tuberculosis. METHODS: Probes were designed according to the sequence of Mycobacterium tuberculosis rpoB gene and the gene array was developed. The DNA fragment which contained hot mutation sites of rpoB gene was amplified with biotin-labelled primers by PCR, and then hybridized with gene...
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