Article
[Rapid detection of Mycobacterium tuberculosis resistance to rifampin using DNA chip].
Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases - 1 Sept 2001
Jing F, Hu Z, Sun Y
Abstract excerpt
OBJECTIVE: To develop a new method, DNA chip, which can be used for rapid detection of Mycobacterium tuberculosis resistance to RFP. METHODS: Designing probe according to the sequence of Mycobacterium tuberculosis rpoB gene and fabricating DNA chip. The DNA fragment which contains hot mutation sites of rpoB gene was labelled with cy5 fluorescence and amplified by PCR technique. Then it was hybridized with DNA...
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