Article
[A new multiplex allele-specific polymerase chain reaction assay for detection of rifampin-resistant Mycobacterium tuberculosis].
Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases - 1 Sept 2005
Jin Jia-lin, Zhang Wen-hong, Hu Zhong-yi, Chen Shu, Weng Xin-hua
Abstract excerpt
OBJECTIVE: To develop a new multiplex allele-specific polymerase chain reaction (MAS-PCR) assay to detect the main mutations in the rifampin resistance dependent region, which has been reported to account for the majority of clinic Mycobacterium tuberculosis resistant to rifampin. METHODS: Based on the sequence of rpoB gene, three specific primers were designed for the MAS-PCR to detect the most common mutations...
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