Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Expression, purification and characterization of recombinant mouse PC6B from baculovirus-infected insect cells.

Protein and peptide letters - 1 Feb 2004

Wang Lie, Yang Guanzhen, Wu Xiangfu

Abstract excerpt

We constructed a mouse PC6B truncated mutant and introduced a tag of 6 consecutive histidines at its carboxyl terminus for simple purification. Using the baculovirus expression system and standard enzymatic assay, we obtained recombinant mouse PC6B protein and with enzymatic activity.

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Baculoviridae
Share to Calcium
Share to Cell Line

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Culture Media

Share to Hydrogen-Ion Concentration

Share to Kinetics

Share to Recombinant Proteins

Share to Spodoptera

Source

Identifiers

PMID: 14965281
DOI: 10.2174/0929866043478509

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 1 Sept 2026. Prepared record publication-14965281-2026-09-01.1. Manifest publication-graph-cohort-2026-09-01.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 1 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Expression, purification and characterization of recombinant mouse PC6B from baculovirus-infected insect cells.PMID 14965281Read ↗

Identification of the role of a cysteine-rich region of PC6B by determining the enzymatic characteristics of its mutants.Semantic proximity Read ↗

Co-expression of the RPS6KB1 and PDPK1 genes for production of activated p70S6K1 using Bac-to-Bac baculovirus expression systemSemantic proximity Read ↗

An improved vector for baculovirus-mediated protein production in mammalian cellsSemantic proximity Read ↗

Improved PC1/3 production through recombinant expression in insect cells and larvae.Semantic proximity Read ↗

Molecular cloning of M6: Identification of a PLP/DM20 gene familySemantic proximity Read ↗

Characterization and Novel Purification of Recombinant Human Protein C from Three Mammalian Cell LinesSemantic proximity Read ↗

Crystal structure of the kinase domain of human protein tyrosine kinase 6 (PTK6) at 2.33 Å resolution.Semantic proximity Read ↗

Molecular cloning and transfection studies of M6b-2, a novel splice variant of a member of the PLP-DM20/M6 gene family.Semantic proximity Read ↗

High-level expression and purification of recombinant SCF ubiquitin ligases.Semantic proximity Read ↗

Expression of recombinant human GM2-activator protein in insect cells: purification and characterization by mass spectrometry.Semantic proximity Read ↗

[Construction and identification of primary open angle glaucoma disease MYOC Pro356Leu mutant plasmid in mouse].Semantic proximity Read ↗

Production and Characterization of Monoclonal antibodies to Xenopus proteinsSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.