Article
Production of recombinant human osteoprotegrin from Trichoplusia ni cells and Bombyx mori larvae.
Protein and peptide letters - 1 Aug 2004
Liu Zhen, Yang Guan-Zhen, Wu Xiang-Fu
Abstract excerpt
Human osteoprotegrin (OPG) and its truncated mutant OPG-280 and lengthened mutant OPG-Fc were constructed and successfully expressed in Trichoplusia ni cells and Bombyx mori larvae. Native SDS-PAGE and Western blot analysis revealed that OPG-Fc is present as a homodimer in Tn cells or B. mori larvae compared with OPG and OPG-280. Furthermore, the hypocalcemic effect assay showed that truncation of the C-terminal...
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