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Article

Impact of antibody framework residue VH-71 on the stability of a humanised anti-MUC1 scFv and derived immunoenzyme.

British journal of cancer - 4 May 2004

Krauss J, Arndt M A E, Zhu Z, Newton D L, Vu B K, Choudhry V, Darbha R, Ji X, Courtenay-Luck N S, Deonarain M P, Richards J, Rybak S M

Abstract excerpt

Anti-MUC1 single-chain Fv (scFv) fragments generated from the humanised antibody huHMFG1 had adequate antigen-binding properties but very poor stability irrespective of the applied linker or domain orientation. Mutagenesis of heavy-chain framework residue V(H)-71, previously described as a key residue for maintaining the CDR-H2 main-chain conformation and thus important for antigen binding, markedly stabilised...

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Share to Antibodies, Monoclonal
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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 15150594
DOI: 10.1038/sj.bjc.6601759
PMCID: PMC2409732

Genes

MUC1

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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