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Article

The influence of the framework core residues on the biophysical properties of immunoglobulin heavy chain variable domains

10 Jan 2009

Abstract excerpt

Antibody variable domains differ considerably in stability. Single-chain Fv (scFv) fragments derived from natural repertoires frequently lack the high stability needed for therapeutic application, necessitating reengineering not only to humanize their sequence, but also to improve their biophysical properties. The human V(H)3 domain has been identified as having the best biophysical properties among human...

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PMID: 19136675
DOI: 10.1093/protein/gzn077

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