Article
Targeting of the dual oxidase 2 N-terminal region to the plasma membrane.
The Journal of biological chemistry - 16 Jul 2004
Morand Stanislas, Agnandji Diane, Noel-Hudson Marie-Sophie, Nicolas Valérie, Buisson Sandrine, Macon-Lemaitre Laetitia, Gnidehou Sédami, Kaniewski Jacques, Ohayon Renée, Virion Alain, Dupuy Corinne
Abstract excerpt
Dual oxidase 2 (Duox2) is a cell surface glycoprotein that probably provides thyroperoxidase with the H2O2 required to catalyze thyroid hormone synthesis. No functional H2O2-generating system has yet been obtained after transfecting Duox2 into non-thyroid cell lines, because it is retained in the endoplasmic reticulum (ER). We investigated the level of maturation of various Duox2 truncated proteins in an attempt...
Topics
- Amino Acid Sequence
- Animals
- Biotinylation
- Blotting, Western
- CHO Cells
- Cell Line
- Cell Membrane
- Cricetinae
- Cysteine
- DNA, Complementary
- Dual Oxidases
- Endoplasmic Reticulum
- Flavoproteins