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Molecular detection of human Plasmodium species using a multiplex real time PCR

14 Jul 2023

Abstract excerpt

Molecular detection methods have revealed higher sensitivity and specificity than conventional microscopy or rapid diagnostic tests for malaria diagnosis. In this study, we implemented, evaluated and validated according to the ISO 15,189 requirements, a multiplex real-time PCR assay to detect and identify the five human malaria parasites. DNA samples were extracted from whole blood or dried blood spots drawn from...

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PMID: 37452123
DOI: 10.1038/s41598-023-38621-9

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