Article
Assembling new Escherichia coli strains by transduction using phage P1.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2011
Moore Sean D
Abstract excerpt
A protocol is described that allows the transfer of genetic material from one Escherichia coli strain to another using bacteriophage P1. P1 transduction can be used to construct new bacterial strains containing multiple alleles, to restore a locus to wild type, to move specific genetic markers from one strain to another, to relocate different mutant genes to a common genetic background, and to evaluate...
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