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Article

X-ray structural and simulation analysis of a protein mutant: the value of a combined approach.

Proteins - 15 May 2004

Mattos Carla, Cohen Justin D, Green David F, Tidor Bruce, Karplus Martin

Abstract excerpt

The effect of the mutation Arg 96 to His on the stability of bacteriophage T4 lysozyme has been previously studied by calorimetric experiments, X-ray crystallography, and free energy simulation techniques. The experimental and calculated values for the difference between the free energy of denaturation of the mutant and the wild type are in reasonable agreement. However, the two approaches led to different...

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Identifiers

PMID: 15103635
DOI: 10.1002/prot.20031

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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